First Molecular Identification of Sarcocystis Buffalonis in Goats Worldwide in Misan Governorate, Iraq

The current study was conducted in Misan Governorate to detect the parasite Sarcocystis spp. by both conventional and molecular methods identify species of this genus goats as intermediate hosts for multiple parasite. Regarding traditional study, rates infection with macroscopic type were low recorded 2% esophageal samples only goats. sizes cysts ranged between 1-10 mm length 2-6 width. Infection microscopic high rates. When comparing efficiency used our diagnose all examined organs isolated from goats, which included digestion pepsin, squeezing, trichinoscopy. It found that method pepsin most efficient diagnosing parasite, followed While squeezing least microscopically. diagnosis parasite's identity at level through gene amplification using polymerase chain reaction (PCR) target segment DNA extracted 18S rRNA 120 4 samples. 87.9% goat samples, showed positive gene; In 100% esophagus recorded, animals had a infection, while diaphragm, tongue heart 90%, 86.7 80%, respectively. results obtained sequence analysis six four (OP474017.1, OP474018.1, OP476699.1 OP474036.1) closely related Malaysian species; S. capracanis accession number (KR155191.1) score percentages 99.50%, 99.01%, 99.76% 99.13%, Finally, two (OP474061.1 OP474062.1) identical Egyptian buffalonis, numbers (KU247913.1) respectively, matching percentage 84.39% 85.64% buffalonis has identified first time globally present study. Keywords: Goats, OP474061.1, OP474062.1.